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Related Experiment Videos

Lactate dehydrogenase in rat mitochondria.

R B Brandt1, J E Laux, S E Spainhour

  • 1Department of Biochemistry and Molecular Biophysics, Medical College of Virginia/Virginia Commonwealth University, Richmond 23298.

Archives of Biochemistry and Biophysics
|December 1, 1987
PubMed
Summary

Lactate dehydrogenase (LDH) activity exists within mitochondria of rat heart, kidney, liver, and lymphocytes. This mitochondrial LDH, particularly LDH-5, shows varied localization and unique isozymes in kidney mitochondria.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Enzymology

Background:

  • L-lactate dehydrogenase (LDH) is primarily known as a cytosolic enzyme.
  • Mitochondrial localization of LDH has been reported but remains incompletely characterized.
  • Understanding LDH compartmentalization is crucial for metabolic pathway analysis.

Purpose of the Study:

  • To investigate the presence and localization of L-lactate dehydrogenase (LDH) activity within isolated mitochondria from various rat tissues.
  • To determine the submitochondrial distribution of LDH and identify specific LDH isozymes in mitochondrial fractions.

Main Methods:

  • Isolation of mitochondrial preparations from rat heart, kidney, liver, and lymphocytes.
  • Assessment of cytosolic contamination using specific marker enzymes.

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  • Digitonin fractionation and bacterial protease treatment to determine LDH localization.
  • Agarose gel electrophoresis for LDH isozyme analysis.

    • Main Results:

    • Persistent LDH activity was detected in mitochondria from heart, kidney, liver, and lymphocytes, with minimal cytosolic contamination.
    • Substantial portions of mitochondrial LDH were located internally (80% in heart/kidney, 70% in lymphocytes, 40% in liver).
    • Cytosolic LDH-5 was found to associate with the outer mitochondrial membrane in liver, kidney, and heart.
    • Kidney mitochondrial preparations exhibited a unique LDH isozyme band not found in corresponding cytosol.

    Conclusions:

    • Mitochondria possess intrinsic LDH activity across multiple rat tissues.
    • LDH exhibits significant intramitochondrial localization, varying by tissue.
    • Tissue-specific LDH isozyme patterns exist within mitochondria, notably in the kidney.