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Sperm immobilization test and quantitative sperm immobilization test using frozen-thawed sperm preparation applied
Yu Wakimoto1, Atsushi Fukui1, Teruhito Kojima1,2
1Department of Obstetrics and Gynecology Hyogo College of Medicine Nishinomiya Japan.
Reproductive Medicine and Biology
|July 15, 2021
Summary
Cryopreserved sperm can be used for the sperm immobilization test (SIT) with computer-aided sperm analysis (CASA) when fresh sperm is unavailable. Both fresh and frozen-thawed sperm yield similar results for diagnosing immunological infertility.
Area of Science:
- Reproductive immunology
- Andrology
- Infertility diagnostics
Background:
- The sperm immobilization test (SIT) with computer-aided sperm analysis (CASA) is a diagnostic method for immunological infertility.
- A limitation of the SIT-CASA method is the consistent availability of high-quality fresh sperm.
Purpose of the Study:
- To compare the diagnostic performance of frozen-thawed sperm versus fresh sperm in the SIT-CASA method.
- To evaluate the feasibility of using cryopreserved sperm for SIT-CASA.
Main Methods:
- Sperm Freeze medium was used to cryopreserve semen samples.
- Density gradient centrifugation was employed to isolate motile sperm from both fresh and frozen-thawed preparations.
- Sperm motility was assessed using CASA after incubating sperm with serum and complement.
Main Results:
- Frozen-thawed sperm demonstrated similar reactivity to sperm-immobilizing antibodies as fresh sperm.
- The CASA system effectively measured motility for both sperm types.
Conclusions:
- Cryopreserved sperm is a viable alternative to fresh sperm for the SIT-CASA assay.
- This finding supports the use of frozen-thawed sperm in diagnosing immunological infertility when fresh samples are not accessible.
