Dynamics of Foliar Responses to O₃ Stress as a Function of Phytotoxic O₃ Dose in Hybrid Poplar

With background concentrations having reached phytotoxic levels during the last century, tropospheric ozone (O₃) has become a key climate change agent, counteracting carbon sequestration by forest ecosystems. One of the main knowledge gaps for implementing the recent O₃ flux-based critical levels (CLs) concerns the assessment of effective O₃ dose leading to adverse effects in plants. In this study, we investigate the dynamics of physiological, structural, and morphological responses induced by two levels of O₃ exposure (80 and 100 ppb) in the foliage of hybrid poplar, as a function of phytotoxic O₃ dose (POD₀) and foliar developmental stage. After a latency period driven by foliar ontological development, the gas exchanges and chlorophyll content decreased with higher POD₀ monotonically. Hypersensitive response-like lesions appeared early during exposure and showed sigmoidal-like dynamics, varying according to leaf age. At current POD_{1_SPEC} CL, notwithstanding the aforementioned reactions and initial visible injury to foliage, the treated poplars had still not shown any growth or biomass reduction. Hence, this study demonstrates the development of a complex syndrome of early reactions below the flux-based CL, with response dynamics closely determined by the foliar ontological stage and environmental conditions. General agreement with patterns observed in the field appears indicative of early O₃ impacts on processes relevant, e.g., biodiversity ecosystem services before those of economic significance - i.e., wood production, as targeted by flux-based CL.