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Xanthine oxidoreductase is present in human synovium
R E Allen1, J M Outhwaite, C J Morris
1Rheumatism Research Wing, Medical School, Birmingham.
Annals of the Rheumatic Diseases
|November 1, 1987
Summary
Inflamed joints may experience reperfusion injury. This study detected xanthine oxidoreductase activity in human synovium, an enzyme linked to oxidative damage, finding higher levels in rheumatoid tissues.
Area of Science:
- Biochemistry
- Orthopedics
- Pathology
Background:
- Mobile inflamed joints may be susceptible to cyclical ischemic reperfusion injury.
- Xanthine oxidoreductase is implicated in oxidative reperfusion injury.
Purpose of the Study:
- To detect xanthine oxidoreductase activity in human synovium.
- To compare enzyme activity between normal and rheumatoid synovia.
Main Methods:
- Human synovial tissues (3 normal, 5 rheumatoid) were analyzed.
- A carbon-14 radioassay was used to measure the conversion of [14C]xanthine to [14C]uric acid.
Main Results:
- Xanthine oxidoreductase activity was detected in all assayed synovial tissues.
- Rheumatoid synovia exhibited a wider range of activity (0.67-305 microU/g) compared to normal synovia (1.2-5.0 microU/g).
Conclusions:
- Xanthine oxidoreductase activity is present in human synovium.
- Elevated or variable xanthine oxidoreductase activity may be associated with rheumatoid arthritis pathogenesis.