Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K
Ribosomes01:27

Ribosomes

9.1K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
9.1K
Ribosomes01:27

Ribosomes

72.0K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
72.0K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

3.7K
3.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.8K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.8K
Termination of Translation01:44

Termination of Translation

26.2K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
26.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic Bone Disease of Prematurity: A Preventable Morbidity in Very Low Birth Weight Infants.

Indian journal of public health·2026
Same author

A Cross-Sectional Comparative Evaluation of the Forced Oscillation Technique and Spirometry in Patients Suspected of Having Obstructive Airway Disease at a Tertiary Care Centre.

Cureus·2026
Same author

Pulmonologist-performed Ultrasound-guided Transthoracic Biopsy of Pleural-based Lung Masses: Diagnostic Yield and Safety, a Retrospective Study.

Thoracic research and practice·2026
Same author

Co-Administration of LPC and LPS Enhanced the Spinal Cord Vulnerability in a Mouse Model of Focal Demyelination.

Journal of molecular neuroscience : MN·2026
Same author

Exploring Bakuchiol as an HSP90-Targeting Lead Against Triple-Negative Breast Cancer: Evidence from In Silico, In Vitro, and Synergy Studies.

Journal of computer-aided molecular design·2026
Same author

Strength-ductility synergy in lightweight aluminium alloys with nano-layered fibres and core-shell nano-particles.

Nature communications·2026

Related Experiment Video

Updated: Oct 28, 2025

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

11.7K

Quantitative Modeling of Protein Synthesis Using Ribosome Profiling Data.

Vandana Yadav1, Inayat Ullah Irshad2, Hemant Kumar3

  • 1Department of Physics, Indian Institute of Technology Madras, Chennai, India.

Frontiers in Molecular Biosciences
|July 15, 2021
PubMed
Summary

Accurate protein synthesis prediction relies on translation initiation and codon translation rates. This review analyzes methods using ribosome profiling data to extract these rates, aiding gene expression regulation studies.

Keywords:
codon translation ratecodon usage biasquantitative modeling of protein synthesisribo-seqribosome profilingtranslation-initiation rate

More Related Videos

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.7K
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

69.1K

Related Experiment Videos

Last Updated: Oct 28, 2025

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

11.7K
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

8.7K
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

69.1K

Area of Science:

  • Molecular Biology
  • Computational Biology
  • Bioinformatics

Background:

  • Quantitative prediction of protein synthesis necessitates precise translation initiation and codon translation rates.
  • Ribosome profiling data offers insights into ribosome occupancy along transcripts, enabling rate parameter extraction.

Purpose of the Study:

  • To provide a comprehensive analysis of methods for extracting translation rate parameters from ribosome profiling data.
  • To discuss the application of these methods in understanding mRNA features that regulate gene expression.

Main Methods:

  • Detailed analysis of established methods for calculating translation initiation rates.
  • Examination of techniques for determining codon translation rates from ribosome profiling data.
  • Review of computational approaches utilizing these rate parameters.

Main Results:

  • Identification and comparison of key methodologies for extracting translation and initiation rates.
  • Demonstration of how these rates elucidate the role of mRNA features in gene expression.
  • Highlighting the utility of accurate rate parameters in computational modeling.

Conclusions:

  • Ribosome profiling data is crucial for obtaining accurate translation rate parameters.
  • These parameters are vital for deciphering mRNA-based gene expression regulation.
  • Accurate rate parameters enhance computational models of protein synthesis, offering new insights into gene expression kinetics.