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Replication of mumps virus in mouse: transient replication in lung and potential of systemic infection
M Tsurudome1, A Yamada, M Hishiyama
1Department of Microbiology, Mie University School of Medicine, Japan.
Abstract:
A mumps virus strain, which replicated in mouse lung after aerosol inhalation, was obtained by selective replication of a wild strain in L929 cells and by further passaging in mice by intraperitoneal inoculation. All of infected mice survived and rechallenge of the survived mice with the same virus resulted in no virus growth in the lung. Treatment of infected mice with antiserum against interferon (IFN) or asialo GM1 delayed virus clearance from lung. Mice at 5 weeks of age were also sensitive to the virus as well as those at 1 week. When injected intravenously, the virus could grow not only in lung but also in salivary glands, heart and spleen. Furthermore, the virus replicated in liver, spleen, pancreas and testis after intraperitoneal inoculation. Antibody response of mice infected by aerosol inhalation was slower than that of intraperitoneally infected ones in either IgG or IgM production. These results indicated that the adapted virus replicated in mouse lung by a natural route of infection and had a potential to cause systemic infection in mouse.
Insights
This study adapted a mumps virus strain for mouse lung infection via aerosol. The adapted virus replicated systemically, and immune treatments affected viral clearance, indicating potential for mumps infection models.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Mumps virus infection typically occurs via respiratory droplets.
- Establishing effective animal models is crucial for studying mumps pathogenesis and developing countermeasures.
Purpose of the Study:
- To adapt a wild-type mumps virus strain for efficient replication in the mouse lung following aerosol inhalation.
- To investigate the systemic spread and immune response elicited by the adapted mumps virus in mice.
Main Methods:
- Selective replication of a wild mumps virus strain in L929 cells and subsequent passaging in mice via intraperitoneal inoculation.
- Aerosol inhalation exposure to assess lung tropism and systemic infection.
- Intravenous and intraperitoneal inoculation to determine tissue distribution.
- Treatment with antiserum against interferon (IFN) or asialo GM1 to evaluate the role of innate immunity in viral clearance.
- Analysis of antibody responses (IgG and IgM) following different infection routes.
Main Results:
- An adapted mumps virus strain successfully replicated in the mouse lung after aerosol inhalation.
- Infected mice survived and developed resistance to rechallenge, with no detectable virus in the lungs.
- Interferon (IFN) or asialo GM1 antiserum treatment delayed lung virus clearance.
- The virus exhibited tropism for salivary glands, heart, spleen, liver, pancreas, and testis following systemic inoculation.
- Aerosol-infected mice showed a delayed antibody response compared to intraperitoneally infected mice.
Conclusions:
- The adapted mumps virus strain effectively replicates in the mouse lung via a natural infection route.
- The adapted strain has the potential to cause systemic mumps infection in mice.
- This adapted strain provides a valuable model for studying mumps virus pathogenesis and host immune responses.