Growth of Staphylococcus aureus Using a Rotary Cell Culture System

The Rotary Cell Culture System (RCCS) is an apparatus that was originally designed by NASA engineers to simulate microgravity conditions for growth of both eukaryotic and bacterial cell cultures. The RCCS growth environment is also characterized by low fluid shear stress, thereby also providing an in vitro growth condition relevant to certain in vivo environments encountered during bacterial infection. This chapter describes a method for growing Staphylococcus aureus under simulated microgravity conditions using the RCCS and disposable High Aspect Ratio Vessels (HARVs). Small samples can be removed and replaced with fresh media during the experiment (continuous sampling method) or the whole culture can be removed at the end of the experiment (end-point sampling method) for larger sample volumes required for follow-up studies such as RNAseq or proteomics.