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A Rat Model of Orthopedic Implant-Associated Infection for Identification of Staphylococcal Biofilm Proteins
Mei G Lei1, Ravi Kr Gupta1,2, Chia Y Lee3
1Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 15, 2021
Abstract:
Secreted bacterial proteins are difficult to identify directly from an infection site due to a limited amount of bacteria and presence of a large quantity of host proteins. Here we describe a rat model of orthopedic implant that allows us to harvest bacterial biofilm materials sufficient for identification of bacterial proteins in the biofilm matrix by liquid chromatography-tandem MS (GeLC-MS/MS) analysis.
Keywords:
BacteriaBiofilm matrix proteinsOrthopedic implant-associated infectionProteomicsStaphylococcus aureus
