Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Staphylococcus aureus</i> can degrade exogenous fatty acids through β-oxidation.

mBio·2026
Same author

An anti-adhesive compound modulating the production of Staphylococcus aureus cell wall-anchored proteins.

Nature communications·2026
Same author

Characterizing the <i>Staphylococcus aureus</i> fatty acid degradation operon.

Journal of bacteriology·2025
Same author

Gut commensal Bifidobacterium-derived extracellular vesicles modulate the therapeutic effects of anti-PD-1 in lung cancer.

Nature communications·2025
Same author

NKG2D ligand expression on NK cells induces NKG2D-mediated cross-tolerization of cytokine signaling and reduces NK cell tumor immunity.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same author

Progesterone receptor-dependent downregulation of MHC class I promotes tumor immune evasion and growth in breast cancer.

Journal for immunotherapy of cancer·2025

Related Experiment Video

Updated: Oct 28, 2025

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses
06:53

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

2.3K

Bone Marrow-Derived Macrophage Infection Assay.

Miranda J Ridder1, Mary A Markiewicz1, Jeffrey L Bose2

  • 1Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, KS, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 15, 2021
PubMed
Summary

This chapter details methods for producing macrophages from mouse bone marrow and conducting infection assays. These techniques help study bacterial pathogen virulence and host-pathogen interactions in vitro.

Keywords:
Bone marrowInfectionIntracellularMacrophageStaphylococcus

More Related Videos

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
07:12

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Published on: June 23, 2023

5.4K
Bone Marrow-derived Macrophage Production
07:06

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

74.3K

Related Experiment Videos

Last Updated: Oct 28, 2025

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses
06:53

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

2.3K
Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
07:12

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Published on: June 23, 2023

5.4K
Bone Marrow-derived Macrophage Production
07:06

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

74.3K

Area of Science:

  • Microbiology
  • Cell Biology
  • Immunology

Background:

  • Cultured mammalian cells offer a simplified model for studying bacterial pathogens and host-pathogen interactions.
  • Macrophage infection assays are crucial for investigating the molecular mechanisms of bacterial pathogenesis.
  • In vitro models serve as alternatives or prescreens for in vivo virulence studies.

Purpose of the Study:

  • To provide a detailed protocol for generating macrophages from mouse bone marrow.
  • To describe the methodology for performing macrophage infection assays.
  • To facilitate the study of bacterial virulence and host-pathogen interactions using cell culture models.

Main Methods:

  • Isolation and differentiation of primary macrophages from mouse bone marrow.
  • Establishment of bacterial cultures for infection assays.
  • Detailed procedures for conducting macrophage infection experiments and subsequent analysis.

Main Results:

  • The described methods enable the successful production of functional macrophages.
  • The protocols allow for reproducible infection assays to study bacterial-macrophage interactions.
  • This approach provides a valuable tool for assessing bacterial virulence potential.

Conclusions:

  • Macrophage infection assays using cultured cells are a powerful tool for studying bacterial pathogenesis.
  • The described methods offer a reliable and accessible approach for researchers in microbiology and immunology.
  • This chapter serves as a practical guide for implementing these essential in vitro techniques.