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Long non-coding RNA MIAT impairs neurological function in ischemic stroke via up-regulating microRNA-874-3p-targeted

Shuai Zhang1, Yue Zhang2, Na Wang1

  • 1Department of Neurology, the Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.

Brain Research Bulletin
|July 15, 2021
PubMed
Abstract

Insights

Myocardial infarction-associated transcript (MIAT) impairs neurological function in ischemic stroke by up-regulating microRNA-874-3p and interleukin-1B. Targeting MIAT or IL-1B, or promoting miR-874-3p, improves outcomes in stroke models.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) show promise for diagnosing and treating ischemic stroke (IS).
  • The role of myocardial infarction-associated transcript (MIAT) in IS, particularly its interaction with microRNA-874-3p (miR-874-3p) and interleukin-1B (IL-1B), requires further investigation.

Purpose of the Study:

  • To evaluate the diagnostic and therapeutic potential of MIAT in ischemic stroke.
  • To elucidate the molecular mechanism involving MIAT, miR-874-3p, and IL-1B in IS.

Main Methods:

  • Serum levels of MIAT, miR-874-3p, and IL-1B were measured in IS patients.
  • A middle cerebral artery occlusion (MCAO) mouse model was used to assess the effects of manipulating MIAT, miR-874-3p, and IL-1B levels.
  • Neurological function, brain damage, neuronal apoptosis, and inflammation were evaluated in MCAO mice.

Main Results:

  • In IS patients, MIAT and IL-1B were upregulated, while miR-874-3p was downregulated.
  • MIAT sponges miR-874-3p, and miR-874-3p targets IL-1B.
  • Modulating MIAT, miR-874-3p, or IL-1B levels significantly impacted neurological function, brain damage, and inflammation in MCAO mice.

Conclusions:

  • MIAT exacerbates neurological deficits in ischemic stroke by upregulating IL-1B through miR-874-3p inhibition.
  • MIAT, miR-874-3p, and IL-1B represent potential therapeutic targets for ischemic stroke.
Keywords:
Behavioral activityInterleukin 1BIschemic strokeLong non-coding RNA myocardial infarction-associated transcriptNeurological functionmicroRNA-874-3p

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