Rhizosphere interactions between PAH-degrading bacteria and Pteris vittata L. on arsenic and phenanthrene dynamics...

The pot experiment was conducted to monitor the dynamics in soil solution chemistry in order to determine the main rhizosphere processes determining As and PAH bioavailability when utilizing P. vittata and PAH-degrading bacteria to remediate co-contaminated soils. The result showed that P. vittata was capable of depleting soil solution As and increasing phenanthrene solubilization, and thus facilitating plant As uptake and phenanthrene dissipation. Bacterial inoculation enhanced soil phenanthrene dissipation and concurrently modified As bioavailability though increasing soil pH, facilitating Fe and Ca minerals solubilization, and accelerating organic matter decomposition. However, the main factors that determine As bioavailability in the rhizosphere considerably varied with plant genotypes. Upon bacterial inoculation, P and Fe strongly influenced As(V) availability and its uptake by the Guangxi accession, and DOC, Fe, and pH were the main parameters correlated with As(V) availability in the rhizosphere of the Hunan accession. Bacterial inoculation tended to stimulate As(V) reduction in the rhizosphere of P. vittata. Microbial-induced changes in Ca, S, and C cycling and pH were indicators of As(V) reduction. Although bacterial inoculation increased soil As and phenanthrene availability, striking differences in As and nutrients uptake and phenanthrene dissipation were observed between P. vittata genotypes. It is suggested that apart from the microbial transformation, plant genotypes and bacterial mediated plant nutrition are also the critical factors in controlling the fates of As and phenanthrene. Our results uncovered the interactions between P. vittata and PAH-degrading bacteria on rhizosphere properties and nutrients cycling regulating As and PAH availability and remediation efficiency.