Real-time visualization of oxyradical burst from single neutrophil by using ultrasensitive video intensifier
1Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Biochemical and Biophysical Research Communications
|December 31, 1987
Abstract:
Dynamics of oxyradical burst from single human neutrophils stimulated by opsonized zymosan was visualized by using an ultrasensitive video intensifier microscopy in the presence of chemiluminescence probe. Luminol-dependent photonic burst activities were clearly corresponding to the distribution of zymosan-treated neutrophils. Heterogeneity of photon-bursting period and the maximum photonic intensity among reactive cells was demonstrated. This may reflect functional heterogeneity of the ability to release oxyradicals among neutrophils.


