Related Experiment Video

Updated: Oct 28, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.9K

MicroRNA-25-3p promotes cisplatin resistance in Non-small-cell lung carcinoma (NSCLC) through adjusting PTEN/PI3K/AKT

Butong Sun1, Nanjun Hu1, Dan Cong1

  • 1Department of Hematology and Oncology, China-Japan Union Hospital of Jilin University, Changchun City, China.

Bioengineered
|July 16, 2021
PubMed

Insights

MicroRNA miR-25-3p increases cisplatin resistance in non-small cell lung cancer (NSCLC). Targeting this microRNA and the PTEN/PI3K/AKT pathway may overcome chemotherapy resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Drug resistance is a major challenge in non-small cell lung cancer (NSCLC) treatment.
  • MicroRNAs play significant roles in regulating cellular processes, including drug resistance.

Purpose of the Study:

  • To investigate the role of miR-25-3p in cisplatin (DDP) resistance in NSCLC.
  • To elucidate the underlying molecular mechanisms, including the PTEN/PI3K/AKT pathway.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for miR-25-3p expression.
  • Cell viability (MTS assay), colony formation, apoptosis (Annexin V/PI), and invasion (Transwell) assays.
  • Luciferase reporter, Western blot, and in vivo tumor growth studies.

Main Results:

  • miR-25-3p expression was elevated in cisplatin-resistant NSCLC cells.
  • miR-25-3p mimic enhanced DDP resistance, invasion, and metastasis, activating the PTEN/PI3K/AKT pathway.
  • PTEN was identified as a direct target of miR-25-3p; PTEN inhibition mimicked miR-25-3p effects.
  • In vivo studies confirmed miR-25-3p promotes tumor growth and DDP resistance.

Conclusions:

  • The miR-25-3p/PTEN/PI3K/AKT axis promotes cisplatin resistance in NSCLC.
  • This axis represents a potential therapeutic target for overcoming chemotherapy resistance in NSCLC.
Keywords:
NSCLCPTEN/PI3K/AKTcisplatin resistancemiR-25-3p

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.6K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.6K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.1K