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Updated: Oct 28, 2025

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
Published on: September 11, 2014
Insight into diatom frustule structures using various imaging techniques
Izabela Zgłobicka1, Jürgen Gluch2, Zhongquan Liao2
1Faculty of Mechanical Engineering, Bialystok University of Technology, Białystok, Poland. i.zglobicka@pb.edu.pl.
Abstract:
The diatom shell is an example of complex siliceous structure which is a suitable model to demonstrate the process of digging into the third dimension using modern visualization techniques. This paper demonstrates importance of a comprehensive multi-length scale approach to the bio-structures/materials with the usage of state-of-the-art imaging techniques. Imaging of diatoms applying visible light, electron and X-ray microscopy provide a deeper insight into the morphology of their frustules.
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