Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does ethanol inhibit LH secretion in the rat?

J Ellingboe1, D G Shaw, A S Skupny

  • 1Alcohol and Drug Abuse Research Center, McLean Hospital-Harvard Medical School, Belmont, Massachusetts 02178.

Alcohol and Alcoholism (Oxford, Oxfordshire). Supplement
|January 1, 1987
PubMed
Summary

Ethanol

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical predictors of donor antibody titre and correlation with recipient antibody response in a COVID-19 convalescent plasma clinical trial.

Journal of internal medicine·2020
Same author

Lipids in shallow bottom sediments.

Environmental science & technology·2012
Same author

The return of science.

History and theory·2011
Same author

Novel (4-piperidin-1-yl)-phenyl sulfonamides as potent and selective human beta(3) agonists.

Bioorganic & medicinal chemistry·2001
Same author

New oxadiazolidinedione derivatives as potent and selective human beta3 agonists.

Bioorganic & medicinal chemistry letters·2001
Same author

(4-Piperidin-1-yl)phenyl amides: potent and selective human beta(3) agonists.

Journal of medicinal chemistry·2001

Area of Science:

  • Neuroendocrinology
  • Reproductive Endocrinology
  • Pharmacology

Background:

  • Acute ethanol doses are believed to suppress LHRH and LH secretion in rats.
  • This effect, not seen in primates, was attributed to species differences in ethanol administration routes.
  • Previous studies suggested endogenous opioid peptides mediate ethanol's inhibition of LH secretion.

Purpose of the Study:

  • To investigate whether stress, rather than ethanol itself, mediates the inhibition of LH secretion in male rats.
  • To compare the effects of oral/intragastric ethanol administration versus intraperitoneal injection on LH secretion and stress markers.
  • To explore the role of the hypothalamic-pituitary-adrenocortical (H-P-A) axis in ethanol-induced neuroendocrine changes.

Main Methods:

  • Male rats received ethanol via intragastric (ig) or intraperitoneal (ip) routes.
  • Intracerebroventricular (icv) injection of a corticotropin-releasing factor (CRF) antagonist was used in some experiments.
  • Measurements included luteinizing hormone (LH), prolactin (PRL), and corticosterone levels.

Main Results:

  • Intragastric ethanol administration did not inhibit LH secretion under stress-free conditions.
  • Intraperitoneal ethanol injection significantly inhibited LH secretion and increased PRL and corticosterone, indicating a stress response.
  • Pre-treatment with a CRF antagonist blocked the inhibitory effects of ip ethanol on LH and corticosterone.

Conclusions:

  • The inhibition of LHRH and LH secretion by ethanol in rats may be primarily due to stress induced by the administration method, not ethanol itself.
  • Intraperitoneal ethanol administration activates stress pathways (H-P-A axis) that suppress reproductive hormone secretion.
  • Findings suggest a re-evaluation of ethanol's direct neuroendocrine effects versus stress-mediated responses is warranted.

Related Experiment Videos