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Ethanol-induced modulation of 35S-TBPS binding to brain membranes
S Liljequist1, S Culp, B Tabakoff
1Laboratory for Studies of Neuroadaptive Processes, NIAAA, Bethesda, MD 20892.
Alcohol and Alcoholism (Oxford, Oxfordshire). Supplement
|January 1, 1987
Abstract:
The effect of ethanol in vitro on the binding of 35S-t-butylbicyclophosphorothionate to brain membranes was examined. Ethanol inhibited basal binding of 35S-TBPS both in potassium bromide- (KBr)- and potassium chloride (KCl)-containing assay media. Ethanol was, however, considerably weaker in reducing 35S-TBPS binding in buffers with 100 mM KCl. Ethanol had no effect on the inhibition of 35S-TBPS binding produced by GABA and pentobarbital in the presence of KBr or KCl, whereas it clearly altered the enhancement of 35S-TBPS binding produced by these drugs when the assay was carried out in buffers containing KCl.