Related Experiment Video
Updated: Oct 28, 2025
![Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F60445.jpg&w=3840&q=50)
04:12
Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate DMBA-TPA
Published on: December 19, 2019
14.8K
Tracking the risk factors associated with high-risk cSCC: A 10-year, Two-Institution, Greek study
Emmanouil Dimonitsas1, Gregory Champsas, Despoina Kakagia
11Department of Plastic and Reconstructive Surgery, Greek Anticancer Institute, Saint Savvas Hospital, Athens, Greece.
Summary
Tumor size, poor differentiation, and invasion depth are key factors predicting outcomes in high-risk cutaneous squamous cell carcinoma (cSCC). This study identified prognosticators for recurrence, metastasis, and death, aiding future treatment strategies.
Area of Science:
- Oncology
- Dermatology
- Surgical Pathology
Background:
- Cutaneous squamous cell carcinoma (cSCC) is a common skin cancer with potential for aggressive behavior.
- Identifying prognostic factors for high-risk cSCC is crucial for effective management and improved patient outcomes.
- Current staging and risk stratification systems require refinement for optimal clinical application.
Purpose of the Study:
- To identify independent risk factors for sentinel lymph node biopsy (SLNB) positivity, local recurrence (LR), metastasis (M), and death caused by cSCC (DCS) in high-risk patients.
- To compare the Brigham and Women's Hospital (BWH) system with a previous Greece-based system for cSCC risk stratification.
- To propose a novel classification system for high-risk cSCC based on identified prognostic factors.
Main Methods:
- A cohort of 1,524 high-risk cSCC patients was analyzed retrospectively from January 2004 to December 2014.
- Univariate and multivariate Cox logistic regression models were employed to assess potential risk factors for SLNB, LR, M, and DCS.
- Data from two medical institutions were pooled for comprehensive analysis.
Main Results:
- Key prognostic factors for LR included tumor diameter ≥2 cm, poor differentiation, incomplete excision, and perineural invasion.
- Factors predicting metastasis (M) and death (DCS) encompassed high-risk tumor site, tumor diameter ≥2 cm, poor differentiation, invasion beyond subcutaneous tissue, incomplete excision, perineural invasion, and recurrence.
- Among patients undergoing SLNB, 25% had positive results, with an 11% false-negative rate.
Conclusions:
- Tumor diameter, differentiation, invasion depth, and perineural invasion are significant independent prognostic factors for high-risk cSCC.
- The findings support the critical role of tumor diameter in cSCC staging and prognosis.
- This research provides a foundation for developing new clinical trials, adjuvant therapies, and improved tumor staging incorporating SLNB.
