Anther Culture in Sweet Pepper (Capsicum annuum L.)

Peppers have a prominent role in traditional cuisine of many different countries all around the world. This is why pepper is one of the most important crops worldwide. Production of doubled haploid (DH) pepper plants has been assessed by different approaches, but at present, the most efficient and universal method is by far anther culture, based on the use of the Dumas de Vaulx et al. protocol published in 1981, and adapted to the particularities of each specific pepper background. In this chapter, we present a method to produce pepper DHs by anther culture, based on the Dumas de Vaulx et al. protocol, but including a number of modifications which, in our experience, allow for a more efficient production DH plants in different pepper genotypes.