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Species with Haploid or Doubled Haploid Protocols.

Jose M Seguí-Simarro1, Javier Belinchón Moreno2, Marina Guillot Fernández2

  • 1Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain. seguisim@btc.upv.es.

Methods in Molecular Biology (Clifton, N.J.)
|July 16, 2021
PubMed
Summary

This updated compilation details 384 species with published methods for producing haploids and doubled haploids (DHs). It includes novel techniques and provides full protocols for 43 species, advancing plant breeding research.

Keywords:
AndrogenesisAnther cultureGynogenesisHaploid inducersIntraspecific hybridizationIrradiated pollenMicrospore cultureOvary cultureOvule cultureSpontaneous haploidsUniparental genome eliminationWide hybridization

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Area of Science:

  • Plant Science
  • Genetics
  • Agricultural Biotechnology

Background:

  • Previous compilations of haploid and doubled haploid (DH) production methods exist, with the last major update in 2003.
  • Advancements in plant breeding necessitate updated information on DH production across diverse species.

Purpose of the Study:

  • To provide a comprehensive, updated list of species with published haploid and/or doubled haploid production methods.
  • To document novel methodologies and include species not covered in prior compilations.
  • To offer detailed protocols for DH production in a subset of these species.

Main Methods:

  • Compilation and critical review of published literature on haploid and DH production.
  • Inclusion of novel techniques and methodologies developed since 2003.
  • Development and presentation of detailed, practical protocols for DH production in selected species.

Main Results:

  • The updated list encompasses 384 species and interspecific hybrids with reported haploid and/or DH production.
  • New species and advanced methodologies have been incorporated since the 2003 compilation.
  • Full protocols for DH production are provided for 43 species, with an additional protocol for a new species.

Conclusions:

  • This work significantly expands the knowledge base for haploid and DH production in plants.
  • The provided protocols facilitate the application of DH technology in a wider range of species.
  • This resource is invaluable for researchers and breeders aiming to utilize haploid and DH technology for crop improvement.