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Tertiary structure in N-linked oligosaccharides.

S W Homans1, R A Dwek, T W Rademacher

  • 1Department of Biochemistry, University of Oxford, England.

Biochemistry
|October 6, 1987
PubMed
Summary
This summary is machine-generated.

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Substitutions on N-linked oligosaccharide cores influence Man alpha 1-3Man beta linkage orientation. Bisecting GlcNAc or xylose residues induce similar conformational transitions, impacting nearby proton resonances.

Area of Science:

  • Carbohydrate Chemistry
  • Structural Biology
  • Biophysics

Background:

  • N-linked oligosaccharides are crucial in biological processes.
  • The pentasaccharide core is a common structural motif.
  • Understanding oligosaccharide conformation is key to their function.

Purpose of the Study:

  • To define the orientation of the Man alpha 1-3Man beta linkage in N-linked oligosaccharides.
  • To investigate the impact of substitutions on oligosaccharide conformation.
  • To correlate structural changes with chemical shift perturbations.

Main Methods:

  • Two-dimensional nuclear Overhauser effect (2D-NOE) measurements for distance constraints.
  • Analysis of seven N-linked oligosaccharides with a common pentasaccharide core.

Related Experiment Videos

  • Molecular orbital energy calculations.

    • Main Results:

    • Conformational invariance of the Man alpha 1-3Man beta linkage was observed in structures with substitutions on the antenna.
    • Bisecting GlcNAc or xylose residues induced similar conformational transitions.
    • These transitions correlated with chemical shift perturbations near the Man alpha 1-3Man beta linkage.

    Conclusions:

    • Oligosaccharide core substitutions, specifically bisecting GlcNAc or xylose, can significantly alter Man alpha 1-3Man beta linkage conformation.
    • Conformational changes are likely driven by local energetic constraints.
    • Modulation of oligosaccharide solution conformations has functional implications.