Interaction of different-sized ZnO nanoparticles with maize (Zea mays): Accumulation, biotransformation and...

This study aimed to investigate the biotransformation of ZnO nanoparticles (NPs) in maize grown in hydroponics for ecotoxicity assessment. Maize seedlings grown for 14 days were exposed to a solution of 9 nm ZnO NPs, 40 nm ZnO NPs, and ZnSO₄ at a Zn concentration of 300 mg L^-1 for 1, 3, and 7 days, respectively. The results of in-situ Zn distribution in maize (Zea mays) showed that 9 nm ZnO NPs could quickly enter the roots of maize and reach the center column transport system of the stem. The results of transmission electron microscopy combined with energy dispersive X-ray spectroscopy revealed that ZnO NPs were accumulated in the vacuoles of the roots, and then transformed and transported through vesicles. Simulated studies showed that low pH (5.6) played a critical role in the transformation of ZnO NPs, and organic acids (K_f = 10^11.4) could promote particle dissolution. Visual MINTEQ software simulated the species of Zn after the entry of ZnO NPs or Zn²⁺ into plants and found that the species of Zn was mainly Zn²⁺ when the Zn content of plants reached 200-300 ppm. Considering that the lowest Zn content of the roots in treatments was 1920 mg kg^-1, combination of the result analysis of root effects showed that the toxicity of roots in most treatments had a direct relationship with Zn²⁺. However, treatment with 9 nm ZnO NPs exhibited significantly higher toxicity than ZnSO₄ treatment on day 1 when the Zn²⁺ concentration difference was not significant, which was mainly due to the large amount of ZnO NPs deposited in the roots. To the authors' knowledge, this study was the first to confirm the process of biotransformation and explore the factors affecting the toxicity of ZnO NPs in depth.