Related Experiment Video
Updated: Oct 28, 2025

Inducible and Reversible Dominant-negative DN Protein Inhibition
Published on: January 7, 2019
Reversible Mechanisms of Enzyme Inhibition and Resulting Clinical Significance
Barbara Ring1, Steven A Wrighton2, Michael Mohutsky3
1, Indianapolis, IN, USA.
Abstract:
Inhibition of a drug-metabolizing enzyme by the reversible interaction of a drug with the enzyme, thus decreasing the metabolism of another drug, is a major cause of clinically significant drug-drug interactions. This chapter defines the four reversible mechanisms of inhibition exhibited by drugs: competitive, noncompetitive, uncompetitive, and mixed competitive/noncompetitive. An in vitro procedure to determine the potential of a drug to be a reversible inhibitor is also provided. Finally, a number of examples of clinically significant drug-drug interactions resulting from reversible inhibition are described.
Related Concept Videos
Enzyme Inhibition
Indirect-Acting Cholinergic Agonists: Mechanism of Action
Reversible inhibitors like edrophonium bind to a specific part of the enzyme called the anionic catalytic site. They form noncovalent bonds, which means they are not strongly attached to the enzyme. This creates a temporary and less stable enzyme–inhibitor complex,...
Enzymes
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Feedback Inhibition
Pharmacokinetics: Drug–Drug Interactions
Allosteric Regulation

