Related Experiment Video
Updated: Oct 28, 2025
04:37
Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
781
Platelet activation in critically ill COVID-19 patients
Nader Yatim1,2, Jeremy Boussier1, Richard Chocron3,4
1Translational Immunology Lab, Department of Immunology, Institut Pasteur, 75015, Paris, France.
Annals of Intensive Care
|July 17, 2021
Summary
Platelets significantly contribute to severe COVID-19 outcomes. Elevated soluble P-selectin and specific gene markers like PPBP and SELPLG can predict disease severity and intubation risk.
Area of Science:
- Hematology
- Immunology
- Virology
Background:
- Severe COVID-19 is linked to thrombotic events, but the mechanisms of hemostasis dysregulation are not fully understood.
- Investigating hemostasis is crucial for understanding COVID-19 severity.
Purpose of the Study:
- To identify soluble markers and gene-expression signatures differentiating COVID-19 severity and outcomes.
- To explore the role of platelets in severe COVID-19.
Main Methods:
- Analysis of two independent cross-sectional cohorts.
- Measurement of soluble markers, including sP-selectin.
- Gene-expression profiling and enrichment analysis.
- Receiver operating characteristic (ROC) curve analysis for predictive value.
Main Results:
- Elevated soluble P-selectin (sP-selectin) at admission correlated with COVID-19 severity, predicting intubation and death.
- Gene expression analysis revealed increased platelet activation genes in critically ill patients.
- Upregulation of ITG2AB, GP1BB, PPBP, and SELPLG genes was observed in a grade-dependent manner.
- SELPLG and PPBP showed significant predictive value for intubation.
Conclusions:
- Platelet activation plays a role in severe COVID-19.
- Plasma sP-selectin levels are associated with COVID-19 severity and mortality.
- PPBP/CXCL7 and SELPLG are potential biomarkers for predicting intubation in COVID-19 patients.
Related Concept Videos
01:22Formation of the Platelet Plug
7.9K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
7.9K
01:18Structure and Function of Platelets
2.3K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
2.3K
01:05Introduction to Hemostasis
10.3K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
10.3K
01:09Coagulation
8.5K
The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
8.5K
01:16Clot Retraction and Fibrinolysis
7.5K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
7.5K
01:20Extrinsic and Intrinsic Pathways of Hemostasis
10.3K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
10.3K