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Updated: Oct 28, 2025
Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
Published on: August 5, 2021
Osteogenic effects of microRNA-335-5p/lipidoid nanoparticles coated on titanium surface
Qiang Wang1, Xiaoying Wang2, Paloma Valverde3
1Division of Oral Biology, Tufts University School of Dental Medicine, Boston, MA, USA; Jinan Stomatological Hospital, Jinan, 250001, Shandong, China; Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School and Hospital of Stomatology, Shandong University, 44-1Wenhuaxi Road, Jinan, 250012, Shandong, China.
Objective:
In this study, we aimed to investigate the therapeutic potential of miR-335-5p lipidoid nanocomplexes coated on Titanium (Ti) SLActive surface by lyophilization.
Design:
In our model, we coated miR-335-5p/Lipidoid nanoparticles on titanium implant, seeded GFP-labelled mouse bone marrow stromal cells (BMSCs) onto the functionalized Ti implant surface, and analyzed the transfection efficiency, cell adhesion, proliferation, and osteogenic activity of the bone-implant interface.
Results:
The Ti SLActive surface displayed a suitable hydrophilicity ability and provided a large surface area for miRNA loading, enabling spatial retention of the miRNAs within the nanopores until cellular delivery. We demonstrated a high transfection efficiency of miR-335-5p lipidoid nanoparticles in BMSCs seeded onto the Ti SLActive surface, even after 14 days. Alkaline phosphatase (ALP) activity and cell vitality were significantly increased in BMSCs transfected with miR-335-5p at 7 and 14 days as opposed to cells transfected with negative controls. When miR-335-5p transfected BMSCs were induced to undergo osteogenic differentiation, we detected increased mRNA expression of osteogenic markers including Alkaline phosphatase (ALP), collagen I (COL1), osteocalcin (OCN) and bone sialoprotein (BSP) at 7 and 14 days as compared with negative controls.
Conclusion:
MiR-335-5p lipidoid nanoparticles could be used as a new cost-effective methodology to increase the osteogenic capacity of biomedical Ti implants.
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