One wavelength to excite them all: deep tissue imaging going multicolor
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Updated: Oct 28, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Abstract:
Three-photon fluorescence microscopy has emerged as one of the leading tools for deep tissue imaging in opaque biological specimens. A recent study by Hontani et al. demonstrated a new strategy for multicolor imaging deep in the mouse brain using a single excitation wavelength.
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