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Published on: November 30, 2013
Circular RNA EGLN3 silencing represses renal cell carcinoma progression through the miR-1224-3p/HMGXB3 axis
Gang Zhang1, Jianqiang Wang1, Wei Tan1
1Department of Urinary Surgery, Hanzhong People's Hospital, Hanzhong, Shaanxi, 723000, China.
Background:
Renal cell carcinoma (RCC) is a common tumor of the urinary system, and its global incidence is increasing annually. Circular RNAs (circRNAs) are involved in RCC tumorigenesis; however, the role of circ-EGLN3 (hsa_circ_0031594) derived from the Egl nine homolog 3 (EGLN3) gene in RCC remains undetermined.
Methods:
Circ-EGNL3 expression was examined before and after RNase R and actinomycin treatments in RCC cells and tissues. Cell proliferation, migration, and invasion were assessed using the CCK-8 assay, EdU staining, and wound-healing and Transwell assays. The interactions between microRNA (miR)-1224-3p and circ-EGLN3, and between miR-1224-3p and HMG box domain containing 3 (HMGXB3) were predicted by bioinformatics analysis and validated by dual-luciferase reporter assay.
Results:
Circ-EGLN3 was identified using RNase R and actinomycin treatments. Circ-EGLN3 was upregulated in RCC cells and tissues and correlated with poor overall survival. Silencing of circ-EGNL3 decreased RCC cell proliferation, migration, and invasion. Mechanistic studies indicated that circ-EGNL3 acts as a sponge for miR-1224-3p, which targeted HMGXB3. Circ-EGNL3 indirectly upregulated HMGXB3 by targeting miR-1224-3p, and overexpression of circ-EGLN3 reversed the repressive effects of miR-1224-3p on RCC.
Conclusion:
Circ-EGLN3 regulated RCC progression through the miR-1224-3p/HMGXB3 axis, suggesting its potential as a therapeutic target.
Insights
Circular RNA EGLN3 (circ-EGLN3) promotes renal cell carcinoma (RCC) progression by sponging miR-1224-3p and upregulating HMGXB3. Silencing circ-EGLN3 inhibits RCC cell proliferation, migration, and invasion, indicating its therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Renal cell carcinoma (RCC) incidence is rising globally.
- Circular RNAs (circRNAs) are implicated in RCC tumorigenesis.
- The specific role of circ-EGLN3 in RCC was previously unknown.
Purpose of the Study:
- To investigate the function and mechanism of circ-EGLN3 in renal cell carcinoma (RCC).
- To explore the potential of circ-EGLN3 as a therapeutic target for RCC.
Main Methods:
- Circ-EGLN3 expression analysis in RCC cells and tissues.
- Assessment of RCC cell proliferation, migration, and invasion.
- Dual-luciferase reporter assays to validate interactions between circ-EGLN3, miR-1224-3p, and HMGXB3.
Main Results:
- Circ-EGLN3 expression is upregulated in RCC and correlates with poor survival.
- Silencing circ-EGLN3 inhibits RCC cell proliferation, migration, and invasion.
- Circ-EGLN3 acts as a sponge for miR-1224-3p, indirectly upregulating HMGXB3.
Conclusions:
- Circ-EGLN3 promotes RCC progression via the miR-1224-3p/HMGXB3 pathway.
- Circ-EGLN3 represents a potential therapeutic target for renal cell carcinoma.
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