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Updated: Oct 28, 2025

A New Straightforward Method for Lipophilicity logP Measurement using 19F NMR Spectroscopy
Published on: January 30, 2019
Determination of molar activity of [18F]fluoride by HPLC via sulfonyl derivatization
Dong Zhou1, Sally Schwarz1, John A Katzenellenbogen2
1Department of Radiology, School of Medicine, Washington University in Saint Louis, Saint Louis, MO, 63110, USA.
Abstract:
The molar activity of [18F]fluoride was determined by HPLC of sulfonyl fluorides, which have high UV absorbance and are formed exclusively from sulfonyl chlorides and [18F]fluoride in aqueous solution. The measurable limit of sulfonyl fluorides is as low as 0.1 ppm, allowing measurements up to the theoretical molar activity of [18F]fluoride. The method was validated with standard solutions of fluoride, and provides a convenient way to measure molar activity of [18F]fluoride using only HPLC without specialized equipment.
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