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In Silico Integrative Approach Revealed Key MicroRNAs and Associated Target Genes in Cardiorenal Syndrome
Romana Ishrat1, Mohd Murshad Ahmed1, Safia Tazyeen1
1Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia (A Central University), New Delhi, India.
Bioinformatics and Biology Insights
|July 19, 2021
Summary
This study identifies key microRNAs (miRNAs) and their target genes involved in cardiorenal syndrome (CRS). These findings suggest potential diagnostic biomarkers for this complex condition affecting both heart and kidney function.
Area of Science:
- Biomedical research
- Molecular biology
- Cardiology and Nephrology
Background:
- Cardiorenal syndrome (CRS) involves interconnected heart and kidney dysfunction.
- MicroRNAs (miRNAs) play roles in cardiovascular and kidney diseases and are potential biomarkers.
Purpose of the Study:
- To identify crucial microRNAs (miRNAs) and their target genes in cardiorenal syndrome (CRS).
- To analyze the interactions between these miRNAs and genes.
- To explore their potential as diagnostic biomarkers for CRS.
Main Methods:
- Utilized R/GEO2R tools to identify differentially expressed miRNAs from public datasets (GSE89699, GSE87885).
- Conducted literature mining to retrieve additional relevant miRNAs.
- Constructed miRNA-gene networks using miRNet and analyzed key nodes with CytoHubba (Cytoscape plugin).
- Performed pathway enrichment analysis on overlapped miRNAs.
Main Results:
- Identified three differentially expressed miRNAs (hsa-miR-4476, hsa-miR-345-3p, hsa-miR-371a-5p) from datasets.
- Literature mining yielded 15 miRNAs, with hsa-miR-21-5p found in 8 of the top 10 pathways.
- Five key miRNAs (hsa-mir-122-5p, hsa-mir-222-3p, hsa-mir-21-5p, hsa-mir-146a-5p, hsa-mir-29b-3p) were identified as central nodes in the network.
Conclusions:
- The identified miRNAs and their target genes are likely pathologically relevant in cardiorenal syndrome (CRS).
- These molecules represent promising candidates for future diagnostic biomarker development in CRS.
