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Updated: Oct 28, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

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Published on: June 23, 2022

A Citywide Approach to SARS-CoV2 Testing.

John P Broach^1,2, Monica Lowell¹, Olga Brown¹

¹University of Massachusetts Memorial Health Care, Worcester, MA, United States.

Frontiers in Public Health

|July 19, 2021

Summary

This summary is machine-generated.

A citywide task force in Worcester, Massachusetts, provided accessible COVID-19 testing, reaching over 48,000 individuals. This initiative optimized testing efficiency and addressed disparities during the pandemic.

Keywords:

COVID-19 SARS-CoV2 pandemic public health testing

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Area of Science:

Public Health
Epidemiology
Health Equity

Background:

The COVID-19 pandemic highlighted significant disparities in SARS-CoV-2 testing access and infection rates.
Health systems, city leaders, and community stakeholders in Worcester, MA, recognized the need for equitable testing solutions.

Purpose of the Study:

To establish a citywide Equity Task Force aimed at providing low-barrier SARS-CoV-2 testing to all community members.
To implement and optimize a large-scale, community-based COVID-19 testing program.

Main Methods:

Leveraged state funding from the "Stop the Spread" campaign for widespread testing.
Employed a community-based approach and multiple Plan-Do-Study-Act (PDSA) cycles to refine testing processes.
Optimized operations to achieve a testing capacity of nearly 300 individuals per hour.

Main Results:

Tested over 48,363 individuals between August 2020 and February 2021.
Observed a positivity rate that varied from 1.5% to a high of 13.4% during the study period.
Successfully expanded the scope of care to include population-based SARS-CoV-2 testing amidst pandemic challenges.

Conclusions:

The collaborative effort demonstrated the feasibility of widespread, equitable COVID-19 testing.
Lessons learned from this initiative can inform responses to future infectious disease outbreaks and pandemics.
Community-based strategies are crucial for addressing health disparities during public health crises.