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Detection of the Chilli Leaf Curl Virus Using an Attenuated Total Reflection-Mediated Localized
Sonatan Das1, Dilip Kumar Agarwal2, Bikash Mandal3
1Centre for Research in Nanotechnology and Science, Indian Institute of Technology Bombay, Mumbai 400076, India.
ACS Omega
|July 19, 2021
Summary
A new nanoparticle optical biosensor detects plant virus DNA using localized surface plasmon resonance (LSPR). This gold nanoparticle sensor shows potential for detecting begomoviruses like chilli leaf curl virus.
Area of Science:
- Nanotechnology
- Biosensing
- Plant Virology
Background:
- Plant viruses pose significant threats to agriculture, necessitating sensitive detection methods.
- Optical biosensors offer label-free detection, but require sensitive platforms for viral DNA identification.
Purpose of the Study:
- To develop and validate a nanoparticle-based optical biosensor for plant virus DNA detection.
- To investigate the use of localized surface plasmon resonance (LSPR) for sensing viral DNA.
- To establish an attenuated total reflection (ATR) configuration for enhanced biosensing capabilities.
Main Methods:
- Utilized gold nanoparticles immobilized on an amine-functionalized surface.
- Employed attenuated total reflection (ATR) spectroscopy to monitor localized surface plasmon resonance (LSPR).
- Established the platform as a refractive index sensor and validated with antibody-antigen interactions before detecting viral DNA.
Main Results:
- Demonstrated a functional refractive index sensor based on LSPR peak monitoring.
- Successfully detected chilli leaf curl virus single-stranded DNA using a complementary DNA receptor.
- Achieved a limit of detection of 1.0 μg/mL for the target viral DNA.
Conclusions:
- The developed nanoparticle-based ATR-LSPR system is a viable biosensor for plant virus DNA.
- This nano-biosensor shows significant potential for detecting various begomoviruses.
- The technique offers a sensitive and label-free approach for plant pathogen diagnostics.
