Related Experiment Video
Updated: Oct 27, 2025
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Published on: May 22, 2017
A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
Cristina C Clement1, Padma P Nanaware2, Lawrence J Stern2
1Department of Radiation Oncology, Weill Cornell Medicine, New York, NY 10065 USA.
This study details a new method for quantifying antigen processing within endosomes. The protocol uses mass spectrometry to analyze protein fragments, revealing differences in how dendritic cells process antigens.
Area of Science:
- Immunology
- Cell Biology
- Proteomics
Background:
- Antigen processing by endosomal compartments is crucial for immune responses.
- Understanding protein fragmentation patterns provides insights into immune system function.
Purpose of the Study:
- To describe a novel protocol for detailed quantification of antigen processing.
- To identify differences in endosomal antigen processing by dendritic cells.
Main Methods:
- Incubation of gradient-purified endosomes with antigens.
- Hot spot analysis of tandem mass spectrometry (MS/MS)-sequenced peptides.
- Quantification of protein fragmentation patterns.
Main Results:
- The protocol successfully quantifies antigen processing.
- Differences in endosomal antigen processing by dendritic cells were identified under various experimental conditions.
Conclusions:
- This method offers a detailed approach to studying antigen processing.
- The findings contribute to understanding immune cell responses and antigen presentation.
More Related Videos
08:51The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
Published on: February 12, 2022
08:44Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
Published on: August 3, 2018