Transport of D-beta-hydroxybutyrate across rat liver mitochondrial membranes

1. D-beta-hydroxybutyrate, a major ketone body, is produced or converted in mitochondria from various animal tissues. 2. It is an easy permeate anion of the inner mitochondrial membrane. However, its translocation is not a passive diffusion process since it is inhibited by pyruvate transport inhibitors like alpha-cyanocinnamate and derivatives. 3. This carrier mediated process is associated with proton movements. Besides, dicarboxylate anions strongly inhibit the penetration into mitochondria. 4. This is in agreement with the existence of a second transport process related to the dicarboxylate carrier.