Using UPLC-QTOF/MS and multivariate analysis to explore the mechanism of Bletilla Striata improving PM_2.5-induced...

Exposure to fine particulate matter (PM_2.5) is closely related to lung diseases and has become more and more harmful to public health. The traditional Chinese medicine of Bletilla Striata has the effect of clearing and nourishing the lungs in clinics. The purpose of the study is using metabolomics methods to explore the mechanism of PM_2.5-induced lung injury and Bletilla Striata's therapeutic effect. In this article, we used an Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOF/MS) method to identify the potential biomarkers. The results showed that there were 18 differential metabolites in the plasma and urine of rats with PM_2.5-induced lung injury, involving the glycerophospholipid metabolism pathway, the tryptophan metabolism pathway, and the purine metabolism pathway, etc. After the administration, Bletilla Striata changed the levels of 21 metabolites, and partly corrected the changes in the level of metabolites caused by PM_2.5. The results indicated that Bletilla Striata could exert a good therapeutic effect by reversing the levels of some biomarkers in the rats with PM_2.5-induced lung impairment.