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Pan2-Pan3 complex, together with Ccr4-Not complex, has a role in the cell growth on non-fermentable carbon sources

Shiori Fujii1, Duong Long Duy1, Arvin Lapiz Valderrama2

  • 1Department of Molecular Cell Biology, Graduate School of Comprehensive Human Sciences and Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

Biochemical and Biophysical Research Communications
|July 19, 2021
PubMed

Insights

Investigating yeast deadenylases Ccr4-Not and Pan2-Pan3 in non-fermentable media revealed distinct roles. Deleting both complexes caused severe growth defects, suggesting their crucial involvement in utilizing alternative carbon sources.

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Yeast Biology

Background:

  • * Two major mRNA deadenylase complexes, Ccr4-Not and Pan2-Pan3, regulate gene expression by shortening the poly(A) tail.
  • * Ccr4-mediated deadenylation is crucial for gene expression in yeast stationary phase.
  • * The roles of these deadenylases in different growth conditions, particularly with non-fermentable carbon sources, require further investigation.

Purpose of the Study:

  • * To investigate the impact of deleting both Ccr4-Not and Pan2-Pan3 deadenylase complexes on yeast cell growth in non-fermentable media.
  • * To understand the regulatory mechanisms, such as phosphorylation, of these deadenylases under varying carbon source conditions.
  • * To identify genes that can suppress growth defects associated with the loss of deadenylase function in non-fermentable media.

Main Methods:

  • * Comparative growth analysis of wild-type, ccr4Δ, and ccr4Δ pan2Δ yeast mutants in YPD and Glycerol-Lactate (GlyLac) media.
  • * Phosphorylation analysis of Ccr4, Pan2, and Pan3 proteins in GlyLac medium.
  • * Genetic screening for multicopy suppressors of the ccr4Δ pan2Δ growth defect on YPGlyLac.

Main Results:

  • * While ccr4Δ mutants showed a growth defect in YPD, ccr4Δ pan2Δ mutants exhibited a similar defect. Upon switching to GlyLac medium, only ccr4Δ grew, while ccr4Δ pan2Δ did not.
  • * Ccr4, Pan2, and Pan3 proteins were found to be phosphorylated in GlyLac medium, suggesting post-translational regulation in response to carbon source.
  • * Multicopy suppressors STM1 and REX2 were identified for the ccr4Δ pan2Δ growth defect, encoding a ribosome-associated protein and an RNA exonuclease, respectively.

Conclusions:

  • * The Pan2-Pan3 complex, in addition to the Ccr4-Not complex, plays a significant role in yeast growth on non-fermentable carbon sources.
  • * Phosphorylation likely serves as a regulatory mechanism for deadenylase activity in response to environmental cues like carbon source availability.
  • * The identification of STM1 and REX2 provides insights into cellular pathways that compensate for deadenylase loss during growth on non-fermentable substrates.
Keywords:
Ccr4-notDeadenylaseNon-fermentable carbon sourcePan2-Pan3Saccharomyces cerevisiae

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