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Related Experiment Video

Updated: Oct 27, 2025

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices
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Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

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Best Practices and Progress in Precision-Cut Liver Slice Cultures.

Liza Dewyse1, Hendrik Reynaert2, Leo A van Grunsven1

  • 1Liver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium.

International Journal of Molecular Sciences
|July 20, 2021
PubMed
Summary

Precision-cut liver slices (PCLS) offer a 3D in vitro model for liver disease research. This review evaluates if PCLS limitations, like induced fibrosis, have been overcome since their 1985 invention.

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Area of Science:

  • Hepatology
  • In vitro toxicology
  • Liver disease modeling

Background:

  • Precision-cut liver slices (PCLS) were introduced 35 years ago as a promising in vitro model for liver research.
  • PCLS retain complex 3D liver structures and cell-cell/cell-matrix interactions, mimicking the in vivo environment.
  • A key limitation of PCLS is the procedure-induced fibrotic response.

Purpose of the Study:

  • To review the parameters and potential of PCLS cultures for liver disease research.
  • To critically evaluate if the initial limitations and pitfalls of PCLS have been addressed.
  • To summarize recent advances and assess the progress of PCLS use since 1985.

Main Methods:

  • Literature review of PCLS research.
  • Analysis of PCLS culture parameters and their impact.
Keywords:
3DPCLSchronic liver diseasein vitro liver

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  • Critical evaluation of PCLS advancements and remaining challenges.

    • Main Results:

    • PCLS offer a superior in vitro model due to their preserved 3D architecture and cell interactions.
    • The procedure-induced fibrotic response remains a significant challenge for PCLS models.
    • Significant progress has been made in PCLS research, though challenges persist.

    Conclusions:

    • PCLS remain a valuable tool for studying liver disease in vitro, offering a more physiologically relevant model.
    • Addressing the fibrotic response is crucial for maximizing the reliability of PCLS.
    • Continued research and refinement are essential for the optimal application of PCLS in liver research.