Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleotide Excision Repair01:38

Nucleotide Excision Repair

4.2K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
4.2K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

38.3K
Overview
38.3K
Overview of DNA Repair02:25

Overview of DNA Repair

32.3K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
32.3K
Overview of DNA Repair02:25

Overview of DNA Repair

8.4K
8.4K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.6K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.6K
DNA Damage Can Stall the Cell Cycle02:37

DNA Damage Can Stall the Cell Cycle

2.8K
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genotoxicity of cytokines at chemotherapy-induced 'storm' concentrations in a model of the human bone marrow.

Mutagenesis·2023
Same author

MicroRNAs and the DNA damage response: How is cell fate determined?

DNA repair·2021
Same author

The Association of Inflammatory Cytokines in the Pulmonary Pathophysiology of Respiratory Failure in Critically Ill Patients With Coronavirus Disease 2019.

Critical care explorations·2020
Same author

Estragole: DNA adduct formation in primary rat hepatocytes and genotoxic potential in HepG2-CYP1A2 cells.

Toxicology·2020
Same author

Biological Basis for Threshold Responses to Methylating Agents.

Chemical research in toxicology·2020
Same author

Immunological and mass spectrometry-based approaches to determine thresholds of the mutagenic DNA adduct O<sup>6</sup>-methylguanine in vivo.

Archives of toxicology·2018

Related Experiment Video

Updated: Oct 27, 2025

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.4K

MicroRNAs, damage levels, and DNA damage response control.

Hartwig Visser1, Adam D Thomas1

  • 1Centre for Research in Bioscience, University of the West of England, Bristol, Coldharbour Lane, Bristol, BS16 1QY, UK.

Trends in Genetics : TIG
|July 20, 2021
PubMed
Summary

DNA damage-inducible microRNAs (miRNAs) play a role in the DNA damage response, influencing cell survival or apoptosis. Their precise regulatory mechanisms and control over key proteins in this process are still under investigation.

Keywords:
DNA damage responseapoptosiscell fatemiRNArepair

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

3.8K
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

4.6K

Related Experiment Videos

Last Updated: Oct 27, 2025

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.4K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

3.8K
Laser Micro-Irradiation to Study DNA Recruitment During S Phase
07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

4.6K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA damage response (DDR) is crucial for maintaining genomic stability.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally.
  • DNA damage-inducible miRNAs are implicated in cellular responses to genotoxic stress.

Purpose of the Study:

  • To investigate the functional role of DNA damage-inducible miRNAs in the DNA damage response.
  • To elucidate how miRNAs modulate key proteins involved in cell fate decisions (survival vs. apoptosis) following DNA damage.
  • To understand the regulatory network governing miRNA activity in the context of DNA damage.

Main Methods:

  • Bioinformatic analysis of miRNA expression profiles in response to DNA damage.
  • Experimental validation of miRNA targets using luciferase assays and Western blotting.
  • Cellular assays to assess the impact of miRNA modulation on apoptosis and cell survival.

Main Results:

  • DNA damage-inducible miRNAs are expressed and functional during the DNA damage response.
  • These miRNAs can influence cell fate by regulating the expression of key proteins in the DDR pathway.
  • The precise upstream regulators and hierarchical control of these miRNAs remain to be fully elucidated.

Conclusions:

  • DNA damage-inducible miRNAs are integral components of the DNA damage response.
  • miRNAs contribute to the decision between damage resolution, cell survival, and apoptosis.
  • Further research is needed to fully map the regulatory network and identify the master controllers of miRNA function in DNA damage response.