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Related Concept Videos

Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Histone Modification02:32

Histone Modification

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Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
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Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Related Experiment Video

Updated: Oct 27, 2025

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

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H3K27Ac modification and gene expression in psoriasis.

Moamen Masalha1, Iddo Z Ben-Dov2, Oren Ram3

  • 1Laboratory of Molecular Cell Biology, Center for Cancer Research and Department of Medicine C, Sheba Medical Center, Tel Hashomer, Israel; Faculty of Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

Journal of Dermatological Science
|July 20, 2021
PubMed
Summary

This study reveals distinct histone modifications in psoriatic skin, identifying the GRHL transcription factor as a potential therapeutic target for psoriasis by analyzing H3K27Ac patterns and gene expression.

Keywords:
Acetylation of the histone H3 at lysine 27 (H3K27Ac)Chromatin immunoprecipitation sequencing (ChIP-seq)EpigeneticsPsoriasisTranscription factor

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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Area of Science:

  • Dermatology
  • Epigenetics
  • Molecular Biology

Background:

  • Psoriatic lesions exhibit altered gene expression compared to healthy skin, but the underlying mechanisms are not fully understood.
  • Epigenetic modifications are crucial for gene regulation, yet genome-wide histone modification studies in psoriasis are limited.
  • This research is the first to compare genome-wide histone modifications between psoriatic and healthy skin.

Purpose of the Study:

  • To investigate the pattern of histone H3 lysine 27 acetylation (H3K27Ac) in psoriatic lesions versus uninvolved and healthy skin.
  • To identify novel genes implicated in psoriasis pathogenesis through analysis of H3K27Ac modifications.
  • To explore the role of transcription factors in psoriasis-associated gene expression changes.

Main Methods:

  • Chromatin immunoprecipitation sequencing (ChIP-seq) was employed to analyze H3K27Ac modification patterns.
  • Comparative analysis of H3K27Ac enrichment was performed between psoriatic, uninvolved psoriatic, and healthy skin samples.
  • mRNA arrays were utilized in conjunction with ChIP-seq data to correlate histone modifications with gene expression levels.

Main Results:

  • A distinct pattern of H3K27Ac modification was observed in psoriatic skin compared to control skin.
  • Many genes with increased expression and H3K27Ac enrichment in psoriasis contain potential binding sites for the GRHL transcription factor.
  • While H3K27Ac enrichment correlates with overexpression of certain genes in psoriasis, a loss of this modification did not consistently lead to decreased gene expression.

Conclusions:

  • H3K27Ac enrichment is prevalent in the most overexpressed genes in psoriasis.
  • The transcription factor GRHL plays a significant role in psoriasis pathogenesis.
  • GRHL emerges as a potential novel therapeutic target for psoriasis treatment.