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Knockdown of D-dopachrome Tautomerase Inhibits Cell Proliferation in Human HepG2 Cell Line

Asuka Takahashi1, Takeo Iwata2, Kikuji Yamashita1

  • 1Department of Functional Morphology, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.

Anticancer Research
|July 20, 2021
PubMed
Abstract

Insights

D-dopachrome tautomerase (DDT) promotes HepG2 cell proliferation, similar to macrophage migration inhibitory factor (MIF). Inhibiting DDT reduced cell growth, which was restored by adding recombinant DDT.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • D-dopachrome tautomerase (DDT) is a macrophage migration inhibitory factor (MIF) homolog.
  • DDT promotes tumor cell growth via CD74 and acts as an anti-obesity adipokine.
  • MIF is known to promote HepG2 cell proliferation.

Purpose of the Study:

  • To investigate the role of DDT in HepG2 cell proliferation.
  • To compare the intrinsic properties of DDT and MIF.

Main Methods:

  • HepG2 cells were engineered to express short hairpin RNA targeting the DDT gene.
  • Cell proliferation assays were performed.
  • Gene expression levels, specifically for cyclin D1, were analyzed.

Main Results:

  • Knockdown of DDT inhibited HepG2 cell proliferation.
  • Reduced expression of cyclin D1 was observed in DDT-knockdown cells.
  • Recombinant DDT administration restored the inhibited cell proliferation.

Conclusions:

  • DDT promotes cell proliferation in HepG2 cells.
  • The action of DDT in HepG2 cells may be similar to that of MIF.
Keywords:
Adipokineapoptosiscell proliferationliver cancer

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