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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

High-throughput and single-cell T cell receptor sequencing technologies.

Joy A Pai¹, Ansuman T Satpathy^2,3

¹Program in Immunology, Stanford University School of Medicine, Stanford, CA, USA.

|July 20, 2021

Summary

High-throughput sequencing and single-cell analysis reveal T cell receptor (TCR) diversity. These methods advance understanding of T cell immunity against pathogens and cancer by linking TCRs to cell states.

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Area of Science:

Immunology
Molecular Biology
Genomics

Background:

T cells are crucial for adaptive immunity, recognizing antigens via T cell receptors (TCRs).
The diversity of TCRs enables recognition of numerous foreign antigens but complicates analysis.
Understanding T cell responses is vital for combating pathogens and cancer.

Purpose of the Study:

To review technological advancements in T cell receptor (TCR) sequencing and analysis.
To highlight how these technologies provide insights into T cell-mediated immunity.
To connect TCR sequences with cellular phenotypes.

Main Methods:

High-throughput sequencing for identifying TCR sequences.
Experimental and computational tools for analyzing TCR antigen specificities.
Single-cell technologies to pair TCRs with transcriptional and epigenetic data.

Main Results:

Identification of diverse TCR repertoires in individuals.
Analysis of TCR antigen specificities using advanced tools.
Integration of TCR sequences with single-cell transcriptional and epigenetic profiles.

Conclusions:

Technological advances are revolutionizing the study of T cell immunity.
High-throughput TCR sequencing facilitates a deeper understanding of immune responses.
These methods are key to discovering fundamental insights into T cell function in health and disease.