Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Lines01:16

Cell Lines

9.1K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
9.1K
Methods of Classification and Identification01:28

Methods of Classification and Identification

445
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
445
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

308
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
308

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medical Council of India's Amended Qualifications for Indian Medical Teachers: Well intended, yet half-hearted.

The National medical journal of India·2018
Same author

Characterization of fractional cuts of co-solvent enhanced lignocellulosic fractionation lignin isolated by sequential precipitation.

Bioresource technology·2018
Same author

A case of psychogenic Parkinsonism: Late age of onset should not be a barrier to make the diagnosis.

The Australian and New Zealand journal of psychiatry·2018
Same author

Green synthesis of CuO nanomaterials and their proficient use for organic waste removal and antimicrobial application.

Environmental research·2018
Same author

Nondisseminated rhinosporidiosis with multisite involvement in the head and neck.

Ear, nose, & throat journal·2018
Same author

Robotic nipple-sparing mastectomy: The first experience from Indian subcontinent.

The breast journal·2018
Same journal

Revolutionizing Transcriptomics: From Single-Cell Insights to RNA-based Interventions.

SLAS technology·2026
Same journal

Smartphone-based colorimetric glucose biosensor using peroxidase-like activity of bimetallic catalyst supported onto graphitic carbon nitride nanosheets.

SLAS technology·2026
Same journal

XVCF: Exquisite visualization of VCF data from genomic experiments.

SLAS technology·2026
Same journal

EasyPip: An equipment-agnostic software application to transform automated liquid handlers into efficient walk-up tools for routine plate-based pipetting.

SLAS technology·2026
Same journal

Identification of ubiquitination-related biomarkers in osteoarthritis: Combining transcriptome and Mendelian randomization analysis.

SLAS technology·2026
Same journal

Miniaturization of a Lumit p-ERK immunoassay for cell-based high-throughput screening of a chemogenetic small-molecule library.

SLAS technology·2026
See all related articles

Related Experiment Video

Updated: Oct 27, 2025

Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

54.5K

Traceability Methods for Cell Line Authentication and Mycoplasma Detection.

Kate Dennert1, Rajeev Kumar1

  • 1Advocate Aurora Research Institute Discovery Lab, Aurora Sinai Medical Center, Advocate Aurora Health, Milwaukee, WI, USA.

SLAS Technology
|July 20, 2021
PubMed
Summary
This summary is machine-generated.

Laboratories face challenges with mycoplasma contamination and cell line misidentification. A new surveillance strategy ensures cell line integrity through consistent testing and data tracing for reliable research outcomes.

Keywords:
cell line authenticationcontaminationmisidentificationmycoplasmashort tandem repeat

More Related Videos

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

16.7K
Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
09:15

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Published on: July 12, 2022

5.0K

Related Experiment Videos

Last Updated: Oct 27, 2025

Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

54.5K
Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
13:39

Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory

Published on: July 7, 2023

16.7K
Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction
09:15

Measuring Single-Cell Mitochondrial DNA Copy Number and Heteroplasmy Using Digital Droplet Polymerase Chain Reaction

Published on: July 12, 2022

5.0K

Area of Science:

  • Cell biology
  • Laboratory best practices

Background:

  • Mycoplasma contamination and cell line misidentification are significant challenges in cell culture.
  • These issues compromise research integrity and lead to misleading results.
  • There is a growing need for robust verification of cell lines before publication.

Purpose of the Study:

  • To develop and implement a comprehensive surveillance strategy for cell line authentication and mycoplasma detection.
  • To establish reliable processes for testing, data analysis, and action planning.
  • To ensure the integrity and reliability of patient and commercial cell lines used in research.

Main Methods:

  • Development of standardized protocols for mycoplasma testing.
  • Implementation of cell line authentication procedures.
  • Creation of a data management system for tracking test results and cell line history.

Main Results:

  • Established a proactive surveillance system to detect and manage contamination and misidentification.
  • Enabled consistent monitoring and verification of cell line quality.
  • Protected the integrity of research data derived from cell cultures.

Conclusions:

  • A dedicated surveillance strategy is crucial for maintaining high-quality cell lines.
  • Systematic testing and data tracing safeguard against research errors.
  • Implementing these methods ensures reliable and reproducible scientific findings.