The Hitchhiker's guide to glycoproteomics

Area of Science:

• Biochemistry
• Molecular Biology
• Systems Biology

Background:

• Protein glycosylation is a vital post-translational modification essential for cellular functions.
• Altered glycosylation patterns are indicative of various diseases, highlighting glycoproteins as key biomarkers and therapeutic targets.

Purpose of the Study:

• To provide a starting point for understanding glycoproteomics.
• To review techniques for characterizing single glycoproteins and complex glycoproteomes.
• To introduce strategies for capturing and analyzing glycoprotein data within multi-omics workflows.

Main Methods:

• Characterization techniques for single glycoproteins.
• Technologies for complex glycoproteome analysis.
• Strategies for capturing glycoprotein data from biological samples (tissues, fluids, cells).

Main Results:

• The review covers a range of approaches, methodologies, and technologies in glycoproteomics.
• It details methods for obtaining glycoprotein-specific and system-wide data.
• It discusses the integration of glycoproteomics into multi-omics for comprehensive analysis.

Conclusions:

• Glycoproteomics is a powerful tool for functional analysis of protein glycosylation.
• System-wide glycoproteome characterization is achievable with current technologies.
• Integrating glycoproteomics into multi-omics workflows enhances the identification and characterization of essential glycoproteins.