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Published on: October 14, 2021
CD27 mRNA expression in mycosis fungoides
Ilaria Galliano1, Valentina Daprà1, Renata Ponti2
1Unit of Infectious Diseases, Department of Pediatrics, Regina Margherita Children's Hospital, University of Turin, Turin, Italy.
Background:
The etiopathogenesis of MF remains obscure. CD27 is a member of the tumor necrosis factor receptor superfamily (TNFRS) that regulates lymphocyte function. Expression of CD27 protein and mRNA has been reported in B-cell lymphomas and adult T-cell leukemia/lymphoma. In this study, we examined the expression of CD27 in the skin of MF patients by real time PCR. The amount of CD27 was measured in MF patients and healthy controls.
Methods:
A total of 98 skin biopsies were analyzed: 12 obtained from healthy donors and 86 obtained cryostatic sections OCT-embedded affected by MF. Relative quantification of mRNA CD27 expression was achieved by means of TaqMan (Thermo Fisher Scientific, Waltham, MA, USA) amplification and normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Results:
Housekeeping gene was detectable in all skin samples and there is no difference between healthy control and MF P value =0.1564. CD27 mRNA sequences were found in 3 of 12 (25%) of skin obtained from healthy donors and in 59 of 86 (68%) of skin obtained from cryostatic sections OCT-embedded affected by MF. The χ2 statistic with Yates correction is 6.8413 and the P value is 0.0089. When we compared the CD27 expression in MF and controls the RQ analysis showed a value of 9.12±14.13. A RQ of 9.12 means that this gene is 9.12 times more expressed in MF skin samples then in the healthy skin samples. No difference was observed in the MF clustered by stages.
Conclusions:
Our findings indicates that CD27 can be used as diagnostic/prognostic markers, and whether anti-CD27 antibodies can be used in therapy.
Insights
Increased CD27 mRNA expression in Mycosis Fungoides (MF) skin indicates its potential as a diagnostic and prognostic marker. This finding may inform future anti-CD27 antibody therapies for MF.
Area of Science:
- Immunology
- Dermatology
- Molecular Biology
Background:
- The exact cause of Mycosis Fungoides (MF) is unknown.
- CD27, a tumor necrosis factor receptor superfamily member, regulates lymphocyte function and is expressed in certain lymphomas.
- Previous studies noted CD27 expression in B-cell lymphomas and adult T-cell leukemia/lymphoma.
Purpose of the Study:
- To investigate the expression levels of CD27 mRNA in the skin of MF patients.
- To compare CD27 expression in MF skin samples with healthy controls.
Main Methods:
- Analysis of 98 skin biopsies (12 healthy, 86 MF patients).
- Quantification of CD27 mRNA expression using TaqMan real-time PCR.
- Normalization of CD27 expression to the housekeeping gene GAPDH.
Main Results:
- CD27 mRNA was detected in 25% of healthy skin samples and 68% of MF skin samples.
- CD27 mRNA expression was significantly higher in MF patients compared to healthy controls (P=0.0089).
- On average, CD27 was 9.12 times more expressed in MF skin than in healthy skin.
Conclusions:
- CD27 is significantly overexpressed in the skin of Mycosis Fungoides patients.
- CD27 may serve as a valuable diagnostic and prognostic biomarker for MF.
- The findings suggest potential therapeutic applications for anti-CD27 antibodies in MF treatment.
