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Updated: Oct 27, 2025

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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MicroRNA 452 regulates IL20RA-mediated JAK1/STAT3 pathway in inflammatory colitis and colorectal cancer

Santosh Lamichhane1, Ji-Su Mo2, Grinsun Sharma1

  • 1Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, 54538, Republic of Korea.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|July 20, 2021
PubMed
Abstract

Insights

MicroRNA 452 (MIR452) suppresses colorectal cancer (CRC) and colitis by targeting IL20RA, regulating the JAK1-STAT3 pathway. This suggests MIR452 as a potential therapeutic target for these conditions.

Area of Science:

  • Molecular Biology
  • Oncology
  • Immunology

Background:

  • MicroRNAs (miRNAs) are key regulators in biological processes, including cancer development.
  • MIR452 is upregulated in colorectal cancer (CRC) and colitis, but its functional role remains unclear.
  • Understanding MIR452's targets and pathways is crucial for CRC and colitis research.

Purpose of the Study:

  • To investigate the functional mechanisms of MIR452 in colorectal cancer and a DSS-induced colitis model.
  • To determine if MIR452 regulates IL20RA and its downstream JAK-STAT signaling pathway.
  • To elucidate the role of MIR452 as a potential therapeutic target.

Main Methods:

  • Luciferase reporter assays to confirm MIR452 targeting of IL20RA.
  • Western blot and quantitative RT-PCR to measure gene and protein expression.
  • Immunohistochemistry to assess molecular changes in CRC and colitis models.

Main Results:

  • IL20RA was identified as a direct target gene of MIR452.
  • MIR452 overexpression decreased IL20RA, JAK1, and STAT3 expression in CRC cells.
  • IL20RA knockdown reduced IL20RA, JAK1, and STAT3, but not STAT1 expression.

Conclusions:

  • MIR452 regulates STAT3 via the IL20RA-mediated JAK1 pathway, independent of STAT1.
  • MIR452 functions as a tumor suppressor in human CRC and a mouse colitis model.
  • MIR452 presents a promising therapeutic target for cancer and colitis treatment.
Keywords:
ColitisColorectal cancerIL20RAJAK1MIR452STAT3

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