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Updated: Oct 27, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
MiR-509-3-5p inhibits colon cancer malignancy by suppressing GTSE1
1Department of General Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, 450000, Henan, China.
Background:
The overarching goal of this research was to identify the effect of miR-509-3-5p on colon cancer (CC) and its interaction with potential target gene GTSE1 in CC.
Methods:
The miR-509-3-5p expression was ascertained after performing qRT-PCR analyses, and the ability of GTSE1 to influence this microRNA was detected after carrying out RNA pull-down assay. CCK-8 assay kit was first employed to determine the proliferation of the cells. To examine the migration and invasion level of HCT116 and SW480 cells, cell wound healing and transwell assay were later performed. After constructing luciferase reporter plasmids, luciferase reporter assay was used to confirm the impacts of miR-509-3-5p on GTSE1 in HCT116 and SW480 cells.
Results:
We found that miR-509-3-5p expression reduced in CC, and its overexpression inhibited the proliferation, migration and invasion of CC cells. We later discovered that miR-509-3-5p could target GTSE1 that was then proved to be an oncogene in CC.
Conclusion:
Our study uncovered that miR-509-3-5p regulated CC malignancy by suppressing target gene GTSE1.
Insights
MicroRNA-509-3-5p (miR-509-3-5p) is downregulated in colon cancer (CC). Its restoration suppresses CC cell proliferation, migration, and invasion by targeting the oncogene GTSE1.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Colon cancer (CC) is a significant global health concern.
- Understanding the molecular mechanisms underlying CC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the role of microRNA-509-3-5p (miR-509-3-5p) in colon cancer (CC).
- To explore the interaction between miR-509-3-5p and its potential target gene, GTSE1, in CC.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to assess miR-509-3-5p expression.
- RNA pull-down assay to confirm the interaction between miR-509-3-5p and GTSE1.
- Cell proliferation (CCK-8), migration (wound healing), and invasion (Transwell) assays were performed on CC cell lines (HCT116, SW480).
- Luciferase reporter assay to validate the targeting of GTSE1 by miR-509-3-5p.
Main Results:
- miR-509-3-5p expression was found to be reduced in colon cancer tissues.
- Overexpression of miR-509-3-5p significantly inhibited the proliferation, migration, and invasion of CC cells.
- GTSE1 was identified as a direct target gene of miR-509-3-5p and confirmed to be an oncogene in CC.
Conclusions:
- miR-509-3-5p acts as a tumor suppressor in colon cancer.
- The miR-509-3-5p/GTSE1 axis plays a critical role in regulating CC malignancy.
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