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Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by
Guanqun Chao1, Qianqian Wang2, Fangxu Ye2
1Department of General practice, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China.
Objective:
Investigate the effect and mechanism of berberine on the small intestinal mucosa of non-steroidal anti-inflammatory drugs (NSAIDs) related small intestinal injury.
Materials And Methods:
Twenty-four SD rats were randomly divided into control group, model group and intervention group. The model group and intervention group were treated with diclofenac (7.5 mg/kg·d, 2/d), a total of 4 days tube feeding, and the intervention group was treated with 50 mg/kg·d intragastric administration of berberine after 2 days. The control group was treated with 7.5 mg/kg·d, 2/d 0.9% saline tube feeding. Then we screened differential expression of colonic mucosal gene by the liquid chip technology.
Results:
Compared with the control group, macroscopic and histology score of the model group increased significantly (P < 0.05), HTR4, HTR1a, F2RL3, CALCA, NPY, CRHR2, IL1b, P2RX3, TPH1, HMOX1, TRPV1, VIP, F2RL1, SLC6A4, TFF2, AQP8 content were significantly increased (P < 0.05), NOS1 content decreased significantly (P < 0.05); Compared with the model group, macroscopic and histology score of the intervention group improved significantly (P < 0.05), and HTR4, F2RL3, NPY, CRHR2, IL1b, VIP, AQP8 content were significantly lower (P < 0.05), NOS1 content increased significantly (P < 0.05).
Conclusion:
Berberine has a protective effect on NSAID-associated small intestinal injury, the mechanism may be that berberine decreases the expression of intestinal mucosa HTR4, F2RL3, NPY, CRHR2, IL1b, VIP, AQP8, and increases the expression of NOS1, that to reduce intestinal permeability and protect intestinal mucosal barrier.
Insights
Berberine protects against NSAID-induced small intestinal injury by modulating gene expression. This natural compound reduces intestinal permeability and strengthens the mucosal barrier, offering a potential therapeutic strategy.
Area of Science:
- Gastroenterology and Pharmacology
- Molecular Biology and Toxicology
Background:
- Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used but can cause significant small intestinal injury.
- Understanding the molecular mechanisms underlying NSAID-induced enteropathy is crucial for developing effective treatments.
- Berberine, a natural alkaloid, has shown potential therapeutic properties in various conditions.
Purpose of the Study:
- To investigate the protective effects of berberine on small intestinal mucosa damaged by NSAIDs.
- To elucidate the underlying molecular mechanisms of berberine's action in NSAID-induced small intestinal injury.
Main Methods:
- A rat model of NSAID-induced small intestinal injury was established using diclofenac.
- Rats were divided into control, model, and berberine-treated groups.
- Gene expression in the colonic mucosa was analyzed using liquid chip technology.
Main Results:
- Diclofenac treatment significantly increased macroscopic and histological scores, indicating severe small intestinal injury.
- NSAID-induced injury was associated with altered expression of genes including HTR4, F2RL3, NPY, IL1b, and AQP8.
- Berberine treatment significantly ameliorated the injury, reducing macroscopic and histological scores and normalizing the expression of key genes.
Conclusions:
- Berberine demonstrates a significant protective effect against NSAID-induced small intestinal injury.
- The mechanism involves downregulating the expression of genes such as HTR4, F2RL3, NPY, IL1b, and AQP8, and upregulating NOS1.
- These molecular changes contribute to reduced intestinal permeability and enhanced protection of the intestinal mucosal barrier.
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