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Related Experiment Video

Updated: Oct 27, 2025

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
06:48

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Published on: August 18, 2023

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SpheroidPicker for automated 3D cell culture manipulation using deep learning.

Istvan Grexa1,2, Akos Diosdi1,3, Maria Harmati1

  • 1Synthetic and Systems Biology Unit, Biological Research Centre (BRC), Eötvös Loránd Research Network (ELKH), Temesvári körút 62, Szeged, 6726, Hungary.

Scientific Reports
|July 21, 2021
PubMed
Summary

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A new AI-guided system automates 3D cell culture transfer for cancer research. This innovation standardizes sample preparation, crucial for developing effective anticancer therapies and advancing precision medicine.

Area of Science:

  • Oncology
  • Biotechnology
  • Medical Technology

Background:

  • Cancer affects millions in Europe, with high-throughput screening of cell cultures dominating anticancer therapy research.
  • Patient-derived ex vivo 3D cell cultures are increasingly vital for drug discovery and precision medicine.
  • Standardized sample preparation is a critical unmet need in 3D cell culture screening for drug development.

Purpose of the Study:

  • To introduce an artificial intelligence-guided, low-cost 3D cell culture delivery system.
  • To address the need for standardized and robust sample preparation in 3D cell culture screening.
  • To enhance experimental control and reduce sample variability in cancer research.

Main Methods:

  • Development of an AI-guided system integrating a light microscope, micromanipulator, syringe pump, and computer.

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A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
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Last Updated: Oct 27, 2025

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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

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  • Implementation of morphology-based feature analysis for spheroid selection based on size and shape.
  • Automated transfer of spheroids from standard holders (Petri dishes, microwell plates) to various formats up to 384-well plates.

    • Main Results:

    • The system enables reliable semi- and fully automated spheroid transfer.
    • Morphology-based analysis allows for the selection of uniform spheroids.
    • The device facilitates controlled experimental conditions by minimizing sample variability.

    Conclusions:

    • The AI-guided 3D cell culture delivery system offers a standardized approach to sample preparation.
    • This technology is poised to reduce non-trivial side effects from sample variability in drug discovery.
    • The system represents a significant step towards next-generation precision medicine in oncology.