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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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A pan-cancer analysis of alternative splicing of splicing factors in 6904 patients

Rui Cheng1, Lixing Xiao1, Wenyang Zhou1

  • 1School of Life Science and Technology, Harbin Institute of Technology, Harbin, China.

Oncogene
|July 21, 2021
PubMed

Insights

Alternative splicing of splicing factors is common in cancers, regulating cancer-specific patterns. This study identified 167 factors and developed a database of splicing-derived neoepitopes, highlighting their role in tumorigenesis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genomics

Background:

  • Splicing factors are crucial for gene expression.
  • The role of alternative splicing of splicing factors in cancer remains largely unexplored.

Purpose of the Study:

  • To conduct a pan-cancer analysis of alternative splicing in splicing factors.
  • To identify splicing factors that regulate cancer-specific splicing patterns.
  • To investigate the potential of alternative splicing events as common regulators across cancers.

Main Methods:

  • Pan-cancer analysis of alternative splicing data from 6904 patients across 16 cancer types.
  • Identification of splicing factors involved in cancer-specific splicing.
  • Development of the splicing-derived neoepitopes database (ASPNs).

Main Results:

  • Identified 167 splicing factors with roles in regulating cancer-specific splicing.
  • Abnormal splicing events of splicing factors act as potential common regulators in different cancers.
  • Developed ASPNs database containing putative alternative splicing-derived neoepitopes for 16 cancer types.

Conclusions:

  • Alternative splicing of splicing factors is a common phenomenon across cancer types.
  • This process is involved in pre-RNA splicing and may be an underestimated hallmark of tumorigenesis.
  • Findings provide insights into cancer-specific splicing and potential therapeutic targets.

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