Intra-amniotic Injection of Mouse Embryos
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Published on: January 27, 2011
Liyuan Cui1, Peng Zou1, Qingshuo Meng1
1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Key Laboratory of Medical Molecular Virology of Ministry of Education/Ministry of Health and Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Abstract:
Recent outbreaks of infectious neuro-developmental diseases such as congenital Zika syndrome - have led to a demand for prognosis data from animal models. We developed an intra-amniotic injection mice model that allows Zika virus (ZIKV) infected mice to grow to puberty. In this system, ZIKV is injected into the amniotic fluid of pregnant mice and infected embryos thereafter. ZIKV-infected mice show several symptoms of clinical 'congenital Zika syndrome', including decreased brain volume and mis-laminated retina. We also evaluated several behavioral functions of these ZIKV-infected mice, for example, after the mice reach puberty, they have visual and motor defects. This technique can be used to screen and evaluate drug candidates and may help evaluate the prognosis of infectious neuro-developmental diseases.
