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In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts

Joo Yong Kim1, Hak Soo Seo1,2

  • 1Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.

Bio-Protocol
|July 21, 2021
PubMed

Keywords:
Nitrate reductaseNitrite concentrationin vitro NR activity assay

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
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