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Related Experiment Video

Updated: Oct 27, 2025

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Laminarin Quantification in Microalgae with Enzymes from Marine Microbes.

Stefan Becker1,2, Jan-Hendrik Hehemann1,2

  • 1Center for Marine Environmental Sciences University of Bremen (MARUM), Marine Glycobiology, Bremen, Germany.

Bio-Protocol
|July 21, 2021
PubMed
Summary

Marine beta-glucan laminarin is a key carbon source in algae and the ocean. Researchers developed a new assay using specific enzymes to accurately measure laminarin concentrations in environmental and lab samples.

Keywords:
AlgaeChrysolaminarinDiatomsGlycobiologyGlycoside hydrolaseLaminarinLaminarinaseMarine organic matterβ-Glucan

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Area of Science:

  • Marine biology
  • Biochemistry
  • Microalgal metabolism

Background:

  • Laminarin, a marine beta-glucan, is a primary storage polysaccharide in microalgae.
  • It serves as a crucial carbon and energy source, significantly impacting the marine carbon cycle.
  • Laminarin plays a central role in microalgal energy metabolism.

Purpose of the Study:

  • To develop a method for quantifying laminarin in environmental and laboratory samples.
  • To leverage selective enzymes for precise measurement of this abundant polysaccharide.

Main Methods:

  • Production of three specific laminarin-digesting enzymes via heterologous expression and purification.
  • Hydrolysis of laminarin into glucose and oligosaccharides using these enzymes.
  • Quantification of resulting sugars via a standard reducing sugar assay to determine laminarin concentration.

Main Results:

  • A selective assay for laminarin quantification was successfully developed.
  • The method allows for the measurement of laminarin in concentrated microalgae from seawater and algal cultures.

Conclusions:

  • The developed enzyme-based assay provides a reliable method for monitoring laminarin levels.
  • This tool aids in understanding the role of laminarin in microalgal energy metabolism and marine ecosystems.